Empcr Amplification Method Manual Lib Logo

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Five hundred nanograms of each DNA sample was sheared by nebulization, purified with the MinElute PCR Purification Kit (Qiagen), and subsequently processed according to the GS Rapid Library Preparation Kit Method Manual (Roche/454 Life Sciences, Branford, USA). ..



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Schematic view of library preparation steps A

Supporting Information Glanville et al. 10.1073/pnas.0909775106 SI Text 454Sequencing:RollingCircleAmplificationandShotgunPreparation. The TempliPhi DNA Amplification

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De novo transcriptome analysis using 454 pyrosequencing of

Supporting Information Glanville et al. 10.1073/pnas.0909775106 SI Text 454Sequencing:RollingCircleAmplificationandShotgunPreparation. The TempliPhi DNA Amplification



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SPRIworks –A Simplified Automated Sample Preparation

GS FLX Titanium® MV emPCR® Kit (Lib-L) GS FLX Titanium® Sequencing Kit 96-well plate Instruments Multi-channel Pipettor for use with the Diffinity RapidTip NanoDrop 1000 Spectrophotometer Roche 454 GS FLX or FLX+ Method Amplicon library preparation is performed using the GS Titanium Series Amplicon Library Preparation Method Manual for use with the GS FLX Titanium emPCR (Lib …

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NimbleGen SeqCap EZ Exome Library SR User's Guide

Technical Datasheet: KAPA Lib Quant ILMN_9.17 IfU_1 Tags: optimization and troubleshooting of library construction processes or workflows, Quality control, Quantification of individual libraries or library pools prior to cluster amplification or emPCR, Quantification of libraries prior to and after hybridization capture, Quantification of libraries prior to pooling for multiplexed sequencing

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Supplemental methods Exome capture

Manual evaluation of barcode distributions from the 64 successfully amplified wells reveals that 59 of the wells were monoclonal (predominantly displays one barcode sequence, and this sequence is represented by a major proportion of

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Validation of Next Generation Sequencing Technologies in Comparison to Current Diagnostic Gold Standards for BRAF, EGFRand KRASMutational Analysis

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All of the above steps including amplicon dilution and emPCR procedures can be found in detail in the Amplicon Library Preparation Manual and the emPCR Amplification Method Manual – Lib-A …

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High Resolution HLA Typing of Blood Stem Cell Donors by

Reports Vol. 51 No. 2 2011 128 www.BioTechniques.com throughput sequencing for STR profiling when compared with current CE methods. We present the successful characterization

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High Resolution HLA Typing of Blood Stem Cell Donors by

05996520001 Amplicon Libraries, Lib‐A, Roche, GS FLX Titanium large volume (LV), Roche 148 Applied Science) 25,26 , which allows massive parallel clonal amplification (Figure 2).

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emPCR Amplification Method Manual Lib-L SV Refer to this manual for the procedures. However, if the library yield can be predicted from past experience, one can use a pre-

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Supplemental methods Exome capture

Liability Statements The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd. All trademarks are property of their respective owners.

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Next-generation sequencing (NGS) Applications and Methods

In conclusion, we have demonstrated an automated DNA library preparation method for the GS FLX Titanium instrument that quickly and reproducibly generates higher yields of ssDNA compared to manual execution. Furthermore, the sample throughput of the automated protocol is up to 9 times higher than the manual protocol. The fragment size selection method we describe can be used as a …

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Laughlin MH, Smyk-Randall EM, Novotny MJ, Brown OR, Adams HR. Coronary blood flow and cardiac adenine nucleotides in E. coli endotoxemia in dogs: effects of oxygen radical scavengers. Coronary blood flow and cardiac adenine nucleotides in E. coli endotoxemia in dogs: effects of …

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2007 GPS Data Logger User s Manual itravel Tech 2007/9/10 Contents Read GPS Log From Device... 3 Clear Data Logger Memory... 4 Add Photos... 5 Browse …

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